Lateral buds of Quercus mongolica were taken as explants to study on tissue culture technique .The result showed that the earliest explant differentiation was on the culture medium with MS +6-BA0.2 mg· L-1 +NAA0.01 mg· L-1 +saccharose 30 g· L-1 .The highest budinitiation and growth coefficient was 68.5%on the culture medium with MS +6-BA0.2 mg· L-1 +NAA0.02 mg· L-1 +saccharose 30 g· L-1 , mean height of simple bud was 2.87 cm, proliferation rate was 2.5, the height of the bud is moderate , and the bud was robust .The highest rooting percentage of transgeneration-al seedlings was 60%on the culture medium with 1/2MS+NAA0.05 mg· L-1 +saccharose25 g· L-1 .
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NSTL
万方数据
维普
