Screening and Identification of a Lignin-degrading Fungal Strain and Optimization of Its Culture Medium Components
In order to explore the degradation effect of lignin-degrading fungi on forest surface fuels,surface fuels under broadleaved-Pinus koraiensis forest,Populus davidiana-Betula platyphylla mixed forest,Larix gmelinii-Betula platyphylla mixed forest and Picea koraiensis forest were used as sources of degrading-fungi and degradation test samples.The mass loss rate and lignin degradation rate of fuels after degradation were analyzed.One strain with good ability to degrade lignin in forest surface fuels was screened and identified as Phlebiopsis pilatii.The strain had strong lignin degradation ability.The degradation rates of lignin in fuels under Populus davidiana-Betula platyphylla mixed forest and Picea koraiensis forest were 19.69%and 16.01%after 10 days of treatment.The mass loss rates of fuels under Larix gmelinii-Betula platyphyl-la mixed forest and Picea koraiensis forest were 23.00%and 22.00%after 60 days of treatment.Sucrose,soybean flour and calcium chloride were the optimal carbon source,nitrogen source and inorganic salt for the enzyme production of the strain.The optimized fermentation medium formula with sucrose of 10.0 g·L-1,soybean flour of 20.0 g·L-1,calcium chloride of 3.0 g·L-1,and Tween-80 of 0.5 g·L-1 was determined.
lignin-degrading fungistrain screeningforest surface fuelculture medium optimization
万方数据
维普
