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吉林省龙井保护区野生大豆居群遗传多样性的研究

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利用28对SSR引物对吉林省龙井保护区一个居群的32份野生大豆进行了遗传多样性分析,共检测到120个等位变异,平均等位变异数为4.29个.32份野生大豆的遗传相似系数为0.58~1.0,平均相似系数为0.63.聚类分析结果表明,此居群野生大豆的生长趋势与地理位置有明显的相关性,呈遗传斑块生长.28对SSR引物得到的Simpson指数分布范围为0.119 1~0.673 8,平均值为0.454 4;Shannon-weaver指数分布范围为0.2771~1.478 3,平均值为0.8865.通过遗传多样性指数表明:吉林省龙井保护区此居群的野生大豆具有较高的遗传多样性.
Diversity of a Population of Wild Soybean(G.soja)G rowing in Longjing Conserved Region of Jilin Province
28 SSR markers were used to evaluate the genetic diversity of 32 wild soybean accessions collected from Longjing Conserved Region in Jilin Province.A total of 120 polymorphic bands were amplified with these SSR markers and the average alleles were 4.29 for each marker.The coefficient of genetic similarity among 32 wild soybean accessions ranged from 0.58 to 1.0.the average genetic similarities coefficient was 0.63.Clustering analysis showed that there was a relationship between growth tendency and geographic proximity,and the phenomenon of genetic plaque could be seen clearly.The distribution of Simpson index for 28 SSR loci was 0.1 191~0.6738.the average Simpson index were 0.4544;Shannon-weaver index for 28 SSR loci was 0.277 1~1.4783.the average Shannon-weaver index were 0.8865.These results showed that there existed a highly genetic diversity for wild soybean accessions from LongJing area in Jilin Province.

Wild soybean(G.Soja)PopulationGenetic diversity

孙晓环、刘晓冬、赵洪锟、王玉民、刘宪虎

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延边大学农学院农学系,吉林,龙井,133400

吉林省农业科学院生物技术研究中心,长春,130033

野生大豆 居群 遗传多样性

农业部农业生物资源保护与利用项目农业部CWRC项目

00053198

2010

东北农业科学
吉林省农业科学院

东北农业科学

CSTPCDCSCD
影响因子:0.449
ISSN:1003-8701
年,卷(期):2010.35(2)
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