一株人参内生真菌的鉴定及对人参根腐病的防效
Identification of an Endophytic Fungus from Ginseng and Its Control Effect on Root Rot
张晶晶 1张宁 1侯微 1曲正义 1李亚丽 1郑培和1
作者信息
- 1. 中国农业科学院特产研究所,长春 130112
- 折叠
摘要
对从人参根部分离得到的1株优势内生真菌(编号为MS-01)进行鉴定并研究其对人参根腐病的防效.结合形态学观察及分子生物学的方法对该菌株进行分析鉴定.确定该菌为毛壳菌属的近缘毛壳菌(Ckaetomium subaffine).对峙培养和发酵液抑菌试验结果表明,该菌株对人参根腐病菌具有良好的抑制作用,抑菌率为(61.57±0.68)％,且在该菌株与人参根腐病菌对峙培养的菌丝交界处,人参根腐病菌菌丝出现溶解和断裂.未灭菌的MS-01菌株发酵液对人参根腐病菌的抑菌率为(79.92±0.77)％;灭菌的MS-01菌株发酵液对人参根腐病菌的抑菌率为(47.22±0.39)％.室内盆栽防治试验结果表明,该菌株对人参根腐病具有一定的防治效果.本研究为人参根腐病的生物防治提供了理论基础.
Abstract
A dominant endophytic fungus(MS-01)isolated from the root of ginseng was identified and its antimicro-bial activity was studied.The methods of morphological observation and molecular biology were used.The strain was identified as Chaetomium subaffine.The results of the confrontation culture and the bacteriostatic test of fermenta-tion broth showed that the strain had a good inhibitory effect on ginseng root rot fungus,and the bacteriostatic rate was(61.57±0.68)％.At the junction of the strain and ginseng root rot fungus,the mycelia of ginseng root rot bacteria dissolved and broke.The bacteriostatic rate of unsterilized MS-01 fermentation broth against ginseng root rot was(79.92±0.77)％.The bacteriostatic rate of sterilized MS-01 fermentation broth against ginseng root rot was(47.22± 0.39)％.The results of indoor potted experiment showed that the strain had a certain control effect on ginseng root rot.This study provides a theoretical basis for biological control of ginseng root rot.
关键词
内生真菌/毛壳菌属/近缘毛壳菌/生物防治Key words
Endophytic fungi/Chaetomium/Chaetomium subaffine/Biological control引用本文复制引用
基金项目
国家重点研发计划项目(2017YFC1702102)
中央级公益性科研院所基本科研业务费专项(1610342019015)
中国农业科学院科技创新工程项目(CAAS-ASTIP-2016-ISAPS)
出版年
2024
NETL
NSTL
万方数据