Identification of an Endophytic Fungus from Ginseng and Its Control Effect on Root Rot
A dominant endophytic fungus(MS-01)isolated from the root of ginseng was identified and its antimicro-bial activity was studied.The methods of morphological observation and molecular biology were used.The strain was identified as Chaetomium subaffine.The results of the confrontation culture and the bacteriostatic test of fermenta-tion broth showed that the strain had a good inhibitory effect on ginseng root rot fungus,and the bacteriostatic rate was(61.57±0.68)%.At the junction of the strain and ginseng root rot fungus,the mycelia of ginseng root rot bacteria dissolved and broke.The bacteriostatic rate of unsterilized MS-01 fermentation broth against ginseng root rot was(79.92±0.77)%.The bacteriostatic rate of sterilized MS-01 fermentation broth against ginseng root rot was(47.22± 0.39)%.The results of indoor potted experiment showed that the strain had a certain control effect on ginseng root rot.This study provides a theoretical basis for biological control of ginseng root rot.
Endophytic fungiChaetomiumChaetomium subaffineBiological control