首页|一株人参内生真菌的鉴定及对人参根腐病的防效

一株人参内生真菌的鉴定及对人参根腐病的防效

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对从人参根部分离得到的1株优势内生真菌(编号为MS-01)进行鉴定并研究其对人参根腐病的防效.结合形态学观察及分子生物学的方法对该菌株进行分析鉴定.确定该菌为毛壳菌属的近缘毛壳菌(Ckaetomium subaffine).对峙培养和发酵液抑菌试验结果表明,该菌株对人参根腐病菌具有良好的抑制作用,抑菌率为(61.57±0.68)%,且在该菌株与人参根腐病菌对峙培养的菌丝交界处,人参根腐病菌菌丝出现溶解和断裂.未灭菌的MS-01菌株发酵液对人参根腐病菌的抑菌率为(79.92±0.77)%;灭菌的MS-01菌株发酵液对人参根腐病菌的抑菌率为(47.22±0.39)%.室内盆栽防治试验结果表明,该菌株对人参根腐病具有一定的防治效果.本研究为人参根腐病的生物防治提供了理论基础.
Identification of an Endophytic Fungus from Ginseng and Its Control Effect on Root Rot
A dominant endophytic fungus(MS-01)isolated from the root of ginseng was identified and its antimicro-bial activity was studied.The methods of morphological observation and molecular biology were used.The strain was identified as Chaetomium subaffine.The results of the confrontation culture and the bacteriostatic test of fermenta-tion broth showed that the strain had a good inhibitory effect on ginseng root rot fungus,and the bacteriostatic rate was(61.57±0.68)%.At the junction of the strain and ginseng root rot fungus,the mycelia of ginseng root rot bacteria dissolved and broke.The bacteriostatic rate of unsterilized MS-01 fermentation broth against ginseng root rot was(79.92±0.77)%.The bacteriostatic rate of sterilized MS-01 fermentation broth against ginseng root rot was(47.22± 0.39)%.The results of indoor potted experiment showed that the strain had a certain control effect on ginseng root rot.This study provides a theoretical basis for biological control of ginseng root rot.

Endophytic fungiChaetomiumChaetomium subaffineBiological control

张晶晶、张宁、侯微、曲正义、李亚丽、郑培和

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中国农业科学院特产研究所,长春 130112

内生真菌 毛壳菌属 近缘毛壳菌 生物防治

国家重点研发计划项目中央级公益性科研院所基本科研业务费专项中国农业科学院科技创新工程项目

2017YFC17021021610342019015CAAS-ASTIP-2016-ISAPS

2024

东北农业科学
吉林省农业科学院

东北农业科学

CSTPCD
影响因子:0.449
ISSN:1003-8701
年,卷(期):2024.49(1)
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