Determination of Aristolochic Acid Ⅰ in Different Parts of Asarum by HPLC-UV
The content of aristolochic acid Ⅰ in asarum was determined by ultrasonic extraction with 70％methanol solution and HPLC-UV method.The detection limit was 0.20 ng.The recovery rate was 98.5％-103.5％.The detec-tion method is simple,with good separation,high sensitivity,and good stability.Aristolochic acid Ⅰ was not detected in the roots and stems of asarum,and the content in the leaves was 0.031 3-0.087 3 mg/kg,which was far below the pharmacopoeia limit of 0.001％,and the risk was very low.
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