HPLC-UV法测定细辛不同部位中马兜铃酸Ⅰ的含量
Determination of Aristolochic Acid Ⅰ in Different Parts of Asarum by HPLC-UV
张振都 1邵郅伟 1郝丽珊 1王莹 1李君梅 1张奇 1宋志峰1
作者信息
- 1. 吉林省农业科学院农业质量标准与检测技术研究所/吉林省农业环境与农产品安全重点实验窒,长春 130033
- 折叠
摘要
采用70％甲醇溶液超声提取、HPLC-UV法测定细辛中的马兜铃酸Ⅰ的含量,检出限为0.20 ng,回收率为98.5％～103.5％.检测方法简单,分离度好,灵敏度高,稳定性好.细辛根、茎中未检出马兜铃酸Ⅰ,叶中含量为0.031 3～0.087 3 mg/kg,远低于药典限量要求0.001％,风险很低.
Abstract
The content of aristolochic acid Ⅰ in asarum was determined by ultrasonic extraction with 70％methanol solution and HPLC-UV method.The detection limit was 0.20 ng.The recovery rate was 98.5％-103.5％.The detec-tion method is simple,with good separation,high sensitivity,and good stability.Aristolochic acid Ⅰ was not detected in the roots and stems of asarum,and the content in the leaves was 0.031 3-0.087 3 mg/kg,which was far below the pharmacopoeia limit of 0.001％,and the risk was very low.
关键词
HPLC-UV法/细辛/马兜铃酸ⅠKey words
HPLC-UV/Asarum/Aristolochic acid Ⅰ引用本文复制引用
基金项目
吉林省农业环境与农产品安全重点实验室建设项目(YDZJ202102CXJD031)
出版年
2024
NETL
NSTL
万方数据