摘要
以'新红星'苹果为试材,采用白、紫、黄三种透气膜果袋处理果实,以探究不同颜色果袋引起的光质差异对苹果花青苷合成的影响.结果表明,果实套彩透袋后,其表皮L*值、b*值逐渐降低,a*值逐渐升高,色泽迅速转红;在处理14 d时,以紫透袋果实着色最为深红、L*值最低、a*值最高、b*值最低,其花青苷含量高低为紫透袋＞黄透袋＞白透袋;PAL、DFR、UFGT的活性以紫透袋果实较高,但其CHI的活性却表现相对较低;基因表达分析表明,MdMYB1、MdUFGT、MdUVR8后期的相对表达量均以紫透袋果实最高;相关性分析显示,MdMYB1与紫透袋处理、MdDFR与白透袋处理的果实花青苷含量呈显著正相关.综上表明,套紫透袋能够促进红色苹果花青苷的合成,其效果优于黄透袋和白透袋.
Abstract
In this study,apple fruit of'Starkrimson Delicious'was treated with white,purple and yellow transparent bags.This research aimed to explore the effect of light quality difference caused by different color transparent fruit bags on the anthocyanin synthesis mechanism of apple.The results showed that,after color transparent bag treat-ment,the L*and b*values of the apple skin gradually decreased,the a*value gradually increased,and the color of apple skin turned red quickly.When bagging treated for 14 d,the color was the darkest red,L*value was lowest,a*value was highest,and b*value was lowest of apple fruit in purple bags,at the same time,the anthocyanin content from high to low was purple bags,yellow bags and white bags,successively.The activities of PAL,DFR and UFGT enzyme were higher in purple bag fruits,while the activity of CHI enzyme was relatively low.Analysis of gene ex-pression showed that,the high expression level of MdMYB1,MdUFGT,MdUVR8 all appeared on purple bag fruits in later period.Correlation between MdDFR expression level and the anthocyanin content of apple fruit treated with white bags was significantly related,and the same correlation between MdMYB1 expression level and anthocyanin content of fruit in purple bags as MdDFR gene.In conclusion,purple transparent bag can promote the synthesis of red apple anthocyanin,and its effect is better than yellow transparent bag and white transparent bag.
基金项目
西藏自治区自然科学基金项目(XZ2019ZRG-99)
万方数据