AsA-GSH Cycle Response of Mung Bean to Low Temperature at Initial Flow-ering Stage and the Analysis of Mitigation Effect of Uniconazole
In order to investigate the effects of low temperature stress during the initial flowering stage and pre-spraying with uniconazole(S3307)on leaf AsA-GSH cycle and yield of mung bean(Vigna radiata),Lvfeng 5(L5)and Lvfeng 2(L2)were used as materials in this pot experiment.S3307 was pre-sprayed and treated with continuous low temperature(average 12 ℃)for 5 d and samples were taken every day to analyze the accumulation of reactive oxygen species(ROS),changes in substance content of AsA-GSH cycle,content of osmotic adjustment substances in mung bean leaves and yield.The results showed that the accumulation content of hydrogen peroxide(H2O2)and malondialdehyde(MDA)in mung bean leaves were significantly increased by chilling damage at the initial flowering stage,and the content of ascorbic acid and glutathione were significantly increased.The yield per plant of L5 was sig-nificantly decreased 14.87％-48.14％,and the yield of L2 was significantly decreased 20.60％-56.18％.S3307 has the effect of resisting low temperature stress at the initial flowering stage.Under low temperature conditions,pre-spraying S3307 can effectively increase the soluble sugar content of mung bean leaves,significantly reduce the H2O2 and MDA content,and significantly increase the contents of AsA,DHA and GSH,GSSG.Spraying S3307 has the regulatory effect of increasing the yield of mung bean.Under normal temperature conditions,L5 and L2 in a single plant significantly increased by 4.70％and 7.87％,respectively.Under low temperature conditions,when treated at low temperature for 1-5 d,the yield of single plant increased 5.11％-12.54％,and the yield per plant was signifi-cantly increased 6.30％-32.91％.In conclusion,low temperature during the initial flowering period affected the physiological functions of mung bean leaves such as reactive oxygen species accumulation,AsA-GSH cycle and os-moregulation.Pre-spraying S3307 could effectively alleviate the effects of low temperature stress on mung bean physiology and yield,and this conclusion provides theoretical basis and effective measures for the production of mung bean resistance to low temperature production.
Mung beanUniconazole(S3307)Low temperatureAsA-GSH cycleYield
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