NaCl胁迫对甘蓝型饲料油菜渗透调节物质及Na+、K+含量的影响
Effects of NaCl Stress on Osmotic Adjustment Substances and Na+,K+Con-tents in Brassica napus
王亚娟 1杨洋 1阴法庭 1张凤华1
作者信息
- 1. 石河子大学农学院/新疆兵团绿洲生态农业重点实验室,新疆石河子 832003
- 折叠
摘要
为探究不同NaCl浓度持续盐胁迫下甘蓝型饲料油菜渗透调节物质及Na+、K+含量的变化,采用盆栽试验法,测定不同NaCl浓度持续处理下饲料油菜叶片中的脯氨酸、可溶性糖、可溶性蛋白、甜菜碱、抗氧化酶(超氧化物歧化酶、过氧化物酶、过氧化氢酶)活性,以及植株根、茎、叶不同部位Na+、K+含量的变化.结果表明:随着NaCl浓度的增加,各渗透调节物质发生不同程度的积累,其中以脯氨酸和可溶性糖含量的增加最显著.随着NaCl浓度的增加,抗氧化酶系统中超氧化物歧化酶、过氧化物酶和过氧化氢酶协同作用以清除产生的活性氧,过氧化物酶起主导作用.随NaCl浓度的增加,总体上Na+含量增加K+含量降低,茎中Na+含量先增加后降低.NaCl浓度为250 mmol/L时出现最大值,K+含量在根和叶中变化相对平稳;NaCl浓度为250、350 mmol/L时,茎中K+含量显著降低.NaCl胁迫浓度在250、350 mmol/L时饲料油菜各生理指标发生显著变化,250 mmol/L以上的NaCl胁迫对饲料油菜苗的生长有明显抑制作用.
Abstract
In order to investigate the changes of osmotic adjustment substances and Na+and K+contents in potted rapeseed under different stresses of NaCl concentration,pot experiment was conducted to determine proline,soluble sugar,soluble protein,betaine and antibiotics in rape leaves under different NaCl concentrations.The activities of oxidase(superoxide dismutase,catalase,peroxidase and the changes of Na+and K+contents in different parts of roots,stems and leaves of plants.The results showed that:with the increase of NaCl concentration,the osmotic ad-justment substances accumulaed in different degrees,among which the increase of proline and soluble sugar was the most significant;with the increase of NaCl concentration,SOD,POD and CAT in the antioxidant enzyme system.Synergistic action to remove the generated ROS,POD played a leading role;with the increase of NaCl concentration,the overall Na+content increases K+content,the Na+content in the stem increases first and then decreases,and the maximum value appears when the NaCl concentration was 250 mmol/L.The K+content was relatively stable in roots and leaves.When the NaCl concentration was 250 mmol/L,350 mmol/L,the K+content in the stem decreased sig-nificantly.In conclusion,the physiological indexes of feed rapeseed changed significantly at NaCl stress concentra-tion of 250 mmol/L,350 mmol/L,and NaCl stress above 250 mmol/L significantly inhibited the growth of rapeseed.
关键词
甘蓝型饲料油菜/NaCl胁迫/抗氧化酶/脯氨酸/可溶性糖Key words
Brassica napus/NaC1 stress/Antioxidase/Proline/Soluble sugar引用本文复制引用
基金项目
公益性行业(农业)科研专项项目(201503120)
兵团重大计划项目(2018AA005)
出版年
2024
NETL
NSTL
万方数据