Determination of CBD and THC in Industrial Hemp by HPLC
Based on the optimization of pretreatment conditions and chromatographic separation,a High-performance liquid chromatography HPLC method for the determination of cannabidiol(CBD)and A9-tetrahydro-cannabinol(THC)in industrial hemp was developed.The samples were extracted with 95％ethanol,acetonitrile-water(68:32,v/v)as the mobile phase,and separated by C18 column at 35 ℃ with a flow rate of 1.0 mL/min,the in-jection volume was 10 μL and the detection wavelength was 220 nm.The results show that the method has a good linear relationship in the range of 0.02-0.10 mg/mL,and the correlation coefficients(r)are all greater than 0.998.The detection limits(LOD,S/N=3)and quantitative limits(LOQ,S/N=10)were 0.0001 g/100 g and 0.0003 g/100 g for CBD,0.0006 g/100 g and 0.0018 g/100 g for THC,respectively.The recovery rates of CBD and THC were 92％-101％and 80％-102％,respectively when the negative samples were added with 0.5 mg/kg,50 mg/kg and 200 mg/kg.The method has good accuracy,sensitivity and stability,and can meet the detection requirements of CBD and THC in industrial hemp.
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