Determination of Pyrene in Polluted Soil by Combination of Ultrasonic Extrac-tion and High Performance Liquid Chromatography
The detection and treatment of polycyclic aromatic hydrocarbons in farmland are related to the environ-mental quality of farmland soil and the food security of residents.A method is described for determination of pyrene,a representative pollutant of polycyclic aromatic hydrocarbons in farmland,by high performance liquid chromatogra-phy with ultrasonic extraction.The results showed that the optimal extraction method for the determination of pyrene in farmland soil was 2.00 g soil sample,with dichloromethane-n-hexane(1︰1,V︰V)mixed solvent as the extractant,and 10 mL of extraction solvent was extracted(50 kHz,40 min)sonically twice and concentrated by nitrogen blow-ing at 40 ℃.The results showed that the linear relationship of the pyrene standard curve was good,the correla-tion coefficient was 0.9997,and the detection limit was 0.54 μg/kg.In the range of pyrene concentration of 0.1-50 mg/kg,the average recovery rate of spiked soil was 72.55％-89.66％,and the relative standard deviation was 1.68％-4.27％ .This method has the advantages of simple operation,less time-consuming and less solvent consump-tion,and has practical significance for improving the detection efficiency of pyrene contaminated soil samples and carrying out research on the remediation and treatment of pyrene pollution in farmland.
万方数据
维普
