卡伍尔链霉菌拮抗灰葡萄孢菌最佳发酵条件研究
Study on the Optimal Antagonistic Conditions of Streptomyces cavourensis against Botrytis cinerea
夏蕾 1杨大海 1孙浩翔 1谭笑 1刘柱杉 1张维东 1温嘉伟 1邹晓威2
作者信息
- 1. 吉林省农业科学院农村能源与生态研究所,长春 130033
- 2. 吉林省农业科学院植物保护研究所,长春 130033
- 折叠
摘要
由灰葡萄孢菌(Botrytis cinerea)引起的蔬菜灰霉病是大棚蔬菜常见的严重病害之一,对于该病目前鲜有应用放线菌进行生物防治的研究报道.链霉菌对植物病原菌有拮抗作用,因此可用来作为生防菌开发利用.本研究利用发酵菌液进行平板拮抗试验,确定卡伍尔链霉菌对灰霉病菌有良好的拮抗作用,并进一步对卡伍尔链霉菌产生最大抗菌物质的发酵条件进行测定.结果表明,最佳发酵培养基为基础培养基,最佳接种量为5％～6％,最佳发酵温度为28 ℃,最佳发酵pH值为6～8,最佳发酵培养时间为9～11 d,最佳碳源为蔗糖,最佳氮源为硝酸铵.
Abstract
Vegetable gray mold caused by Botrytis cinerea is one of the most common and serious diseases of green-house vegetables.There are few reports on biological control of this disease by actinomycetes.Streptomyces have an-tagonistic effects on plant pathogens,so they can be used as biocontrol bacteria for development and utilization.In this study,the liquid fermentation of Streptomyces cavourensis was employed to determine the antagonistic effect against B.cinerea via plate confrontation assay,furthermore,the fermentation conditions under which the S.cavou-rensis produced the maximum antibacterial substances were determined.The results showed that the best fermenta-tion medium was basal medium,the best inoculum size was 5％-6％,the best fermentation temperature was 28 ℃,the best fermentation pH value was 6-8,the best fermentation culture time was 9-11 d,the best carbon source was sucrose,and the best nitrogen source was ammonium nitrate.
关键词
灰霉菌/卡伍尔链霉菌/拮抗/生物防治Key words
Botrytis cinerea/Streptomyces cavourensis/Antagonism/Biological control引用本文复制引用
出版年
2024
NETL
NSTL
万方数据