首页|不同pH值对黄瓜枯萎病致病菌生长影响的研究

不同pH值对黄瓜枯萎病致病菌生长影响的研究

扫码查看
黄瓜枯萎病是由尖孢镰刀菌黄瓜专化型(Fusarium.oxysporum f.sp.cucumerinum)侵染所引起的黄瓜病害,明确尖孢镰刀菌的侵染条件对于黄瓜枯萎病的防治具有重要意义.为了探究不同pH值条件对尖孢镰刀菌生长状态的影响,本试验设置了不同pH值梯度的PDA培养基与PDB培养基,观察测定不同培养基中尖孢镰刀菌的生长速度与菌落形态,以期探索最适宜黄瓜枯萎病发生的pH值条件.结果发现黄瓜枯萎病致病菌在PDA培养基中最适条件为pH 5.5~9.0(实际为4.5~6.5),且在不同pH值下的形态也有差异;在PDB培养基中,pH 11处理下的病原菌生长情况最差,这说明菌落不适宜在碱性环境下的PDB培养基中生长.因此,尖镰孢菌适宜在pH 4.5~6.5范围内生长.
Study on the Effect of Different pH Environments on the Growth of Cucumber Fusarium Wilt Pathogen
Cucumber wilt a cucumber disease caused by Fusarium oxysporum f.sp.cucumerinum.Clarifying the en-vironmental conditions under which cucumber wilt disease infects cucumbers is of great significance for the preven-tion and control of cucumber wilt disease.In order to investigate the effect of different pH conditions on the growth status of cucumber wilt pathogen,this experiment configured PDA and PDB media with different pH gradients,ob-served and measured the growth rate and colony morphology of cucumber wilt pathogen in different media,in order to explore the most suitable pH for the occurrence of wilt disease.The results showed that the optimal pH for PDA culture medium was 5.5-9.0(actually 4.5-6.5),and the morphology of the bacteria also varied at different pH levels.In the PDB medium,the growth of the pathogen under pH 11 treatment was the worst compared to other pH treat-ments,indicating that the colony was not suitable for growth in the PDB medium under alkaline conditions.There-fore,Fusarium oxysporum is suitable for growth within the pH range from 4.5 to 6.5.

Fusarium oxysporumpHPDAPDB

李亚航、施艳娥、巴顿、丁卓、崔浩楠

展开 >

河北科技师范学院园艺科技学院/河北省特色园艺种质挖掘与创新利用重点实验室,河北秦皇岛 066000

尖孢镰刀菌 pH值 PDA培养基 PDB培养基

2024

东北农业科学
吉林省农业科学院

东北农业科学

CSTPCD
影响因子:0.449
ISSN:1003-8701
年,卷(期):2024.49(5)
  • 1