Comparison of Three Digital PCR Platforms in DNA Quantitative Detection of Animals and Plants
Digital PCR is an absolute quantitative technique that does not depend on reference material and stan-dard curves,and is widely used in quantitative detection.In this study,three digital PCR platforms,including QX200 droplet digital PCR,Naica droplet chip digital PCR and DropDx-2044 microfluidic digital PCR,were used to determine the specific DNA sequence copy number of animal and plant samples.The throughput and cost of dif-ferent digital PCR platforms were compared,and the characteristics of the three digital PCR platforms in DNA accu-rate quantitative detection were evaluated.The results showed that there were no significant differences in the appli-cability,linear correlation,accuracy and precision of the three digital PCR platforms for the quantitative detection of animals and plants.The Naica digital PCR platform has obvious advantages in terms of detection throughput,detec-tion cost and ease of operation.This study provides a technical reference for laboratories to select an appropriate digital PCR platform for different types of sample detection.
Quantitative detectionApplication ComparisonQX200 droplet digital PCRNaica droplet chip digital PCRDropDx-2044 microfluidic chip digital PCR
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