三个数字PCR平台在动植物DNA定量检测中的应用比较
Comparison of Three Digital PCR Platforms in DNA Quantitative Detection of Animals and Plants
董立明 1闫伟 1邢珍娟 1夏蔚 1刘奕君 2龙丽坤 1李飞武1
作者信息
- 1. 吉林省农业科学院农业质量标准与检测技术研究所,长春 130033
- 2. 吉林农业大学生命科学学院,长春 130118
- 折叠
摘要
数字PCR是一种不依赖外部标准样品和标准曲线的绝对定量技术,在定量检测中应用广泛.本研究针对QX200微滴式数字PCR、Naica微滴芯片数字PCR、DropDx-2044微流控芯片数字PCR等三个数字PCR平台,分别测定动植物样品特异性DNA序列拷贝数.比较不同数字PCR平台的测定结果、检测通量以及检测成本,评价三个数字PCR平台在DNA精准定量检测方面的特性.结果表明:三个数字PCR平台在动植物定量检测的适用性、线性相关性、正确度和精密度等方面均无显著差异;在检测通量、检测成本以及操作简便性方面,Naica数字PCR平台优势明显.本研究对实验室有效控制检测成本以及开展不同类型样品的定量检测提供了技术参考.
Abstract
Digital PCR is an absolute quantitative technique that does not depend on reference material and stan-dard curves,and is widely used in quantitative detection.In this study,three digital PCR platforms,including QX200 droplet digital PCR,Naica droplet chip digital PCR and DropDx-2044 microfluidic digital PCR,were used to determine the specific DNA sequence copy number of animal and plant samples.The throughput and cost of dif-ferent digital PCR platforms were compared,and the characteristics of the three digital PCR platforms in DNA accu-rate quantitative detection were evaluated.The results showed that there were no significant differences in the appli-cability,linear correlation,accuracy and precision of the three digital PCR platforms for the quantitative detection of animals and plants.The Naica digital PCR platform has obvious advantages in terms of detection throughput,detec-tion cost and ease of operation.This study provides a technical reference for laboratories to select an appropriate digital PCR platform for different types of sample detection.
关键词
精准定量检测/应用比较/QX200微滴式数字PCR/Naica微滴芯片数字PCR/DropDx-2044微流控芯片数字PCRKey words
Quantitative detection/Application Comparison/QX200 droplet digital PCR/Naica droplet chip digital PCR/DropDx-2044 microfluidic chip digital PCR引用本文复制引用
出版年
2024
NETL
NSTL
万方数据