HPLC法测定工业大麻中大麻二酚和四氢大麻酚的不确定度评定
Uncertainty Evaluation of the Determination of CBD and THC in Industrial Hemp by HPLC
张鑫 1刘丽宅 1王多伽 1赵云辉 1王蕾 1金香淑 1刘笑笑 1姜晓莉2
作者信息
- 1. 吉林省农业科学院,长春 130033;农业农村部农产品及加工品质量监督检验测试中心(长春),长春 130033
- 2. 吉林省农业科学院,长春 130033
- 折叠
摘要
对采用高效液相色谱法(HPLC)测定工业大麻中大麻二酚(CBD)和四氢大麻酚(THC)的不确定度进行评估,依据《测量不确定度评定与表示》(JJF 1059.1-2012)和《化学分析中不确定度的评估指南》(CNAS-GL006-2019),建立了HPLC法测定工业大麻中CBD和THC的不确定度评定的数学模型,逐层对测定过程中各不确定度来源进行分析,对各不确定度分量进行计算,再对测定结果进行合成不确定度和扩展不确定度评估.结果表明,检测CBD过程中,不确定度来源的主要因素所占分量由大到小排序为:标准溶液配制＞标准工作曲线拟合＞重复次数＞提取＞样品称量;检测THC过程中,不确定度来源的主要因素所占分量由大到小排序为:标准工作曲线拟合＞重复次数＞标准溶液配制＞提取＞样品称量.CBD结果平均值为0.166 g/100g,扩展不确定度为0.031 2 g/100g,取值结果为X=(0.166±0.031 2)g/100g,包含因子k=2;THC平均值为0.052 2 g/100 g,扩展不确定度为0.003 44 g/100 g,取值结果为X=(0.052 2±0.003 44)g/100 g,包含因子k=2.此方法适用于类似条件下CBD和THC测定不确定度的评定.
Abstract
To assess the uncertainty in the determination of cannabidiol(CBD)and Δ9-tetrahydrocannabinol(THC)in industrial cannabis using High-performance liquid chromatography(HPLC),according to'Evaluation and expres-sion of uncertainty in measurement'(JF1059.1-2012)and'Guidelines for the evaluation of uncertainty in chemical analysis'(CNAS-GL006-2019),a mathematical model of uncertainty evaluation for determination of CBD and THC in industrial hemp by HPLC was established.The sources of uncertainty were analyzed and the components of uncer-tainty were calculated,then the synthetic uncertainty and extended uncertainty were evaluated.The results showed that the main factors of uncertainty in the process of CBD were as follows:preparation of standard solution＞fitting of standard working curve＞repeated times＞extraction＞weighing of samples.In the process of THC detection,the main factors of uncertainty were as follows:standard working curve fitting＞times of repetition＞preparation of stan-dard solution＞extraction＞weighing of samples.The average value of CBD was 0.166 g/100 g,the expanded uncer-tainty was 0.0312 g/100 g,the result was X=(0.166±0.0312)g/100 g,the inclusion factor k=2.The average value of THC was 0.0522 g/100 g,the expanded uncertainty was 0.00344 g/100 g,X=(0.0522±0.00344)g/100 g,k=2.This method is suitable for the evaluation of uncertainty of CBD and THC under similar conditions.
关键词
不确定度评定/工业大麻/大麻二酚/四氢大麻酚/高效液相色谱法Key words
Uncertainty evaluation/Industrial hemp/CBD/THC/HPLC引用本文复制引用
出版年
2024
NETL
NSTL
万方数据