Uncertainty Evaluation of the Determination of CBD and THC in Industrial Hemp by HPLC
To assess the uncertainty in the determination of cannabidiol(CBD)and Δ9-tetrahydrocannabinol(THC)in industrial cannabis using High-performance liquid chromatography(HPLC),according to'Evaluation and expres-sion of uncertainty in measurement'(JF1059.1-2012)and'Guidelines for the evaluation of uncertainty in chemical analysis'(CNAS-GL006-2019),a mathematical model of uncertainty evaluation for determination of CBD and THC in industrial hemp by HPLC was established.The sources of uncertainty were analyzed and the components of uncer-tainty were calculated,then the synthetic uncertainty and extended uncertainty were evaluated.The results showed that the main factors of uncertainty in the process of CBD were as follows:preparation of standard solution＞fitting of standard working curve＞repeated times＞extraction＞weighing of samples.In the process of THC detection,the main factors of uncertainty were as follows:standard working curve fitting＞times of repetition＞preparation of stan-dard solution＞extraction＞weighing of samples.The average value of CBD was 0.166 g/100 g,the expanded uncer-tainty was 0.0312 g/100 g,the result was X=(0.166±0.0312)g/100 g,the inclusion factor k=2.The average value of THC was 0.0522 g/100 g,the expanded uncertainty was 0.00344 g/100 g,X=(0.0522±0.00344)g/100 g,k=2.This method is suitable for the evaluation of uncertainty of CBD and THC under similar conditions.
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