Influence of Biochar on Anaerobic Methane Production from Human Feces
In order to enhance the harmless and resourceful utilization of human feces,this study utilized human fe-ces as anaerobic fermentation substrate and pig manure fermentation liquid as inoculum.The effects of biochar de-rived from maize straw,wheat straw,and rice straw,added at proportions of 5%,10%and 20%,were investigated on methane content and cumulative gas production during anaerobic fermentation.The results indicated that under con-stant temperature conditions of 35 ℃ for a fermentation period of 40 days,the highest cumulative gas production was observed in the group with 10%rice straw biochar(C2),followed by the group with 10%wheat straw biochar(B2),and the group with 20%maize straw biochar(A3),with respective values of 6346,5044,4621 mL,respectivly.Both the control and treatment groups exhibited over 99%inactivation of fecal Escherichia coli and mortality of helminth eggs,achieving the objective of harmless treatment.In the anaerobic fermentation system of the biochar-treated groups,advantageous bacterial phyla,Proteobacteria and Synergistetes were detected,indicating a positive impact of biochar on anaerobic methane production from human feces.
Human fecesBiocharAnaerobic fermentationMethaneCumulative gas production