Effect of plant growth retarder B9 on microtuber formation of different varieties of potato
In order to investigate the effect of plant growth retarder B9 on potato in vitro,different varieties of potato virude-free seedlings were used as experimental materials.Under the culture condi-tions of light intensity 2000 Lx,photcycle 16 h·d-1,culture temperature 22℃,MS medium was used as the base medium,and sucrose concentrations of 4%,6%,8%and 10%were set,respectively.After comparing the induction effect of different sucrose concentrations with 3%sucrose concentration,the induced sucrose concentration was determined.Six B9 concentrations of 2mg·L-1,4mg·L-1,6mg·L-1,8mg·L-1 and 10mg·L-1 were set up to determine the average and maximum potato weight of test-tube potato and calculate the large potato rate.Determination of crude protein,reducing sugar and vita-min C in vitro.The results show that:The medium suitable for inducing large potato in vitro was MS+8%sucrose+B9(6mg·L-1).After 30 days of induction,the average single potato weight of Longfeng Black Beauty,Feuritar,Yougin and Yantua 13 varieties was 0.78g,0.76g,0.64g and 0.47g,respec-tively.The maximum potato weight was 0.91g,0.84g,0.78g and 0.68g,respectively.
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