摘要
为探究植物生长延缓剂B9对马铃薯试管大薯的影响,以不同品种的马铃薯脱毒苗为试验材料,在光照强度2 000 Lx、光周期16 h·d-1,培养温度 22℃的培养条件下,以MS培养基为基础培养基,分别设置4%、6%、8%、10%浓度的蔗糖,以3%浓度蔗糖作对照,对比不同蔗糖浓度诱导效果后,确定诱导蔗糖浓度,设置5个B9浓度2 mg·L-1、4 mg·L-1、6 mg·L-1、8 mg·L-1、10 mg·L-1,测定试管薯的平均薯重、最大薯重,统计大薯率,测定试管薯粗蛋白、还原糖与维生素C.结果表明:适合诱导马铃薯试管大薯的培养基为MS+蔗糖8%+B9(6 mg·L-1),诱导30 d,隆丰黑美人、费物瑞它、尤金、延薯13四个品种的平均单薯重分别为0.78 g、0.76 g、0.64 g、0.47 g,最大薯重分别达到0.91 g、0.84 g、0.78 g、0.68 g.
Abstract
In order to investigate the effect of plant growth retarder B9 on potato in vitro,different varieties of potato virude-free seedlings were used as experimental materials.Under the culture condi-tions of light intensity 2000 Lx,photcycle 16 h·d-1,culture temperature 22℃,MS medium was used as the base medium,and sucrose concentrations of 4%,6%,8%and 10%were set,respectively.After comparing the induction effect of different sucrose concentrations with 3%sucrose concentration,the induced sucrose concentration was determined.Six B9 concentrations of 2mg·L-1,4mg·L-1,6mg·L-1,8mg·L-1 and 10mg·L-1 were set up to determine the average and maximum potato weight of test-tube potato and calculate the large potato rate.Determination of crude protein,reducing sugar and vita-min C in vitro.The results show that:The medium suitable for inducing large potato in vitro was MS+8%sucrose+B9(6mg·L-1).After 30 days of induction,the average single potato weight of Longfeng Black Beauty,Feuritar,Yougin and Yantua 13 varieties was 0.78g,0.76g,0.64g and 0.47g,respec-tively.The maximum potato weight was 0.91g,0.84g,0.78g and 0.68g,respectively.
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