In order to investigate the effect of plant growth retarder B9 on potato in vitro,different varieties of potato virude-free seedlings were used as experimental materials.Under the culture condi-tions of light intensity 2000 Lx,photcycle 16 h·d-1,culture temperature 22℃,MS medium was used as the base medium,and sucrose concentrations of 4%,6%,8%and 10%were set,respectively.After comparing the induction effect of different sucrose concentrations with 3%sucrose concentration,the induced sucrose concentration was determined.Six B9 concentrations of 2mg·L-1,4mg·L-1,6mg·L-1,8mg·L-1 and 10mg·L-1 were set up to determine the average and maximum potato weight of test-tube potato and calculate the large potato rate.Determination of crude protein,reducing sugar and vita-min C in vitro.The results show that:The medium suitable for inducing large potato in vitro was MS+8%sucrose+B9(6mg·L-1).After 30 days of induction,the average single potato weight of Longfeng Black Beauty,Feuritar,Yougin and Yantua 13 varieties was 0.78g,0.76g,0.64g and 0.47g,respec-tively.The maximum potato weight was 0.91g,0.84g,0.78g and 0.68g,respectively.
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