In order to create new germplasm of leaf chicory,this article established an EMS mutagenesis technology system for leaf chicory.The results showed that at an EMS concentration of 0.8%and treatment for 6 h,the relative germination rate was close to the half lethal rate,and the induction effect was the best.The seed germination and growth conditions were good,and it was determined as the optimal treatment condition for EMS mutagenesis of leaf chicory.This study established an EMS mutagenesis technology system for leaf chicory,and the research results provide a reference for the construction of a leaf chicory mutant library and the breeding of new varieties.