This paper analyzes the current situation of professional postgraduate talents cultivation in ap-plied undergraduate colleges and universities,and adopts the whole-process OBE concept to apply professional postgraduate talents cultivation in college-enterprise cooperation,and builds a college-enterprise collaborative ed-ucation mode suitable for applied colleges and universities.To explore the joint training system of college-enter-prise-industrial institute mode,and establish the training mode and guarantee system for professional master grad-uates in industrial colleges.Through the research on the training mode of professional graduate students in ap-plied colleges and universities under the industrial institute mode,the practical problems of cultivating high-end applied talents in the development and construction of manufacturing industry can be solved.