Study on Temperature Calibration Method of Isothermal Amplification Rapid Nucleic Acid Detector in Multi-temperature Region
Constant temperature amplification technology can improve the speed of nucleic acid amplification and detection without repeated rise and fall and gentle cycle,and is widely used in nucleic acid POCT equipment.In this paper,based on the structure,temperature control principle and key points of the constant temperature rapid nucleic acid detector in the multi-temperature region,the temperature deviation,heating rate,temperature fluctu-ation and temperature duration deviation of the rapid nucleic acid detector in the multi-temperature region were calibrated and analyzed,and the feasibility and rationality of this method were verified.
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