Research on Calibration Method of Temperature Parameters for 384-hole Polymerase Chain Reaction Analyzer
PCR technology,as a revolutionary molecular biology tool,plays an important role in gene analysis,pathogen detection,drug development,genetic research and other fields.As a representative of high-throughput PCR instrument,the 384 well PCR instrument has the ability to process multiple samples at one time.Because the existing calibration specification is mainly for 96 holes,there are problems of calibration difficulty and poor accura-cy when using 384 holes.Therefore,through the comparison of the two,this paper puts forward the calibration lay-out method of 384-well PCR instrument,and carries out the test verification.The experiment shows that this meth-od is effective.
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