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小通量PCR仪光学扩增模拟器研制及应用

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小通量PCR仪主要用于适应不同应用场景,应对各类应急检测、突发检测等需求,通常采用底部荧光或底侧部荧光采集的检测方式.本文结合其工作原理,区别于常规PCR仪的通量和反应孔分布设计,研制了一款集成温度光学校准功能,可组合使用、小巧便携的光学扩增模拟器,并通过实验验证了该装置的可靠性.
Development and Application of Optical Amplification Simulator for Low-volume PCR Instrument
The low-throughput PCR instrument is mainly used to adapt to different application scenarios and meet the needs of various emergency detection and burst detection,and usually adopts the detection method of bottom fluorescence or bottom side fluorescence collection.In this paper,a compact and portable optical amplification sim-ulator with integrated temperature optical calibration function is developed,which is different from the flux and re-action hole distribution design of conventional PCR instrument.The reliability of the device is verified by experi-ments.

PCR instrumentbottom fluorescence detectionoptical amplification simulatormulti-parameter cali-bration

黄伟俊、王旖、梁林、赵丹侠、郑辉、祝天宇

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台州市计量技术研究院

北京林电伟业电子技术有限公司

PCR仪 底部荧光检测 光学扩增模拟器 多参数校准

2024

计量与测试技术
成都市计量监督检定测试所

计量与测试技术

影响因子:0.175
ISSN:1004-6941
年,卷(期):2024.50(10)