Preparation and Evaluation of Pseudovirus Standards for Ucleic Acid Detection of Three Pathogens
In this paper,the standard products of human immunodeficiency virus,rabies virus and novel coronavir-us were prepared and valued,and the lentivirus system containing specific nucleic acid sequence was used for pseudovirus packaging and gene sequencing,and the uniformity,stability and applicability of the lentivirus were e-valuated by digital PCR method,so as to determine the eigenvalue and extended uncertainty(k=2).The results showed that the nucleic acid sequences of the three pseudoviruses were consistent with those in the NCBI database.
novel coronavirushuman immunodeficiency virusrabies virusdigital PCRfake virus standard
万方数据
维普
