In this paper,the standard products of human immunodeficiency virus,rabies virus and novel coronavir-us were prepared and valued,and the lentivirus system containing specific nucleic acid sequence was used for pseudovirus packaging and gene sequencing,and the uniformity,stability and applicability of the lentivirus were e-valuated by digital PCR method,so as to determine the eigenvalue and extended uncertainty(k=2).The results showed that the nucleic acid sequences of the three pseudoviruses were consistent with those in the NCBI database.
关键词
新型冠状病毒/人类免疫缺陷病毒/狂犬病毒/数字PCR/假病毒标准品
Key words
novel coronavirus/human immunodeficiency virus/rabies virus/digital PCR/fake virus standard
维普
万方数据