摘要
目的 通过试验研究探讨清肝降浊方基于ERS信号蛋白异常表达治疗非酒精性脂肪肝病的作用机制.方法 采用高脂饮食饲喂的方法,诱导建立非酒精性脂肪肝病大鼠动物模型.给药8周后,进行肝功能指标AST、ALT、AKP检测、血清中TC、TG、NEFA及肝组织匀浆中TC、TG检测、肝组织匀浆中SOD、MDA检测,检测信号通路相关蛋白ATF4、eIF2a、CHOP的表达水平.结果 清肝降浊方 865.1 mg/kg、432.6 mg/kg和216.3 mg/kg给药组动物血清AST、ALT、AKP活性及肝组织中ATF4、CHOP表达量、TG含量均明显的低于模型组,而肝组织中SOD活性显著高于模型组(P＜0.01或P＜0.05);865.1 mg/kg和432.6 mg/kg 2个给药组动物血清TC、TG、NEFA含量及肝组织中TC、TG、MDA含量、eIF2a表达量均低于模型组(P＜0.01或P＜0.05).结论 清肝降浊方治疗NAFLD作用机制可能是通过调节ERS信号蛋白异常表达,从而达到防治非酒精性脂肪肝病.
Abstract
Objective To investigate the mechanism of Qinggan Jiangzhuo Formula in the treatment of non-alcoholic fatty liver disease(NAFLD)based on the abnormal expression of ERS signaling proteins.Methods A rat model of nonalcoholic fatty liver disease was induced by high-fat diet feeding.After 8 weeks of drug administration,the liver function indicators AST,ALT,AKP,the levels of serum TC,TG,NEFA,the levels of TC and TG in the liver tissue homogenate,the levels of SOD and MDA in the liver tissue homogenate were detected,and the expression levels of signaling pathway related proteins ATF4,eIF2a,and CHOP were detected as well.Results The activities of AKP,AST,and ALT in the serum,the expressions of ATF4 and CHOP,and the contents of TG in the liver tissue of the animals treated with Qinggan Jiangzhuo Formula at 865.1 mg/kg,432.6 mg/kg,and 216.3 mg/kg were significantly lower than those in the model group,whereas the activity of SOD in the liver tissue was significantly higher(P<0.01 or P<0.05,respectively).The levels of serum TC,TG,and NEFA,as well as the levels of TC,TG,MDA,and the eIF2a expression level in the liver tissue of animals in the two treatment groups of 865.1 mg/kg and 432.6 mg/kg were lower than those in the model group(P<0.01 or P<0.05).Conclusion The mechanism of Qinggan Jiangzhuo Formula in the treatment of NAFLD may be through regulating the abnormal expression of ERS signaling proteins,thereby achieving the prevention and treatment of nonalcoholic fatty liver disease.
基金项目
吉林省卫生健康科技能力提升项目(2021JC071)
维普
万方数据