高效液相色谱法测定益肾排石颗粒中黄芩苷含量
Determination of baicalin in Yishen Paishi granules by HPLC
马永璠 1薄天儒 2于亚杰 3刘艳华 1初洪波1
作者信息
- 1. 长春中医药大学,长春 130117
- 2. 吉林国文医院,吉林 四平 136100
- 3. 四平市产品质量检验院,吉林 四平 136000
- 折叠
摘要
目的 建立益肾排石颗粒中黄芩苷含量的HPLC测定方法,为益肾排石颗粒的质量控制提供科学、可靠依据.方法 采用高效液相色谱法,岛津ODS-2C18 色谱柱(250 mm×4.6 mm,5 μm);流动相为0.2%磷酸水(A)-甲醇(B)梯度洗脱,流速1.0 mL/min,柱温30℃,检测波长280 nm,进样量均为10 μL.结果 该方法黄芩苷峰面积和浓度之间线性关系良好,r=1.000 0;精密度、稳定性均符合要求;平均加样回收率为 103.29%,RSD为 2.38%;三批样品中黄芩苷的平均含量为6.12 mg/g,RSD值为1.71%.结论 本研究方法重复性好,准确度高,可用于复方益肾排石颗粒中黄芩苷的含量测定.
Abstract
Objective To establish an HPLC method for the determination of baicalin in Yishen Paishi granules,and to provide a scientific and reliable basis for the quality control of the medicine.Methods High performance liquid chromatography(HPLC)was performed on Shimadzu ODS-2C18 column(250 mm×4.6 mm,5 μm),with its mobile phase as a gradient elution of 0.2%phosphoric acid water(A)-methanol(B),with its flow rate at 1.0 mL/min,its column temperature at 30℃,its detection wavelength at 280 nm,and its sample size all at 10 μL.Results The linear relationship between the peak area and the concentration of baicalin was good,r=1.000 0.Its precision and stability met the requirements.Its average recovery was 103.29%and RSD was 2.38%.The average content of baicalin in the three batches of samples was 6.12 mg/g,and the RSD value was 1.71%.Conclusion The method has a good repeatability and a high accuracy,and can be used for the determination of baicalin in compound Yishen Paishi granules.
关键词
益肾排石颗粒/黄芩苷/高效液相色谱法/含量测定Key words
Yishen Paishi granules/baicalin/high performance liquid chromatography(HPLC)/content determination引用本文复制引用
基金项目
吉林省科技发展计划(20200404068YY)
吉林省自然科学基金(YDZJ202201ZYTS237)
出版年
2024
国家科技期刊平台
NSTL
万方数据