摘要
目的 研究金振口服液(JZOL)对支气管炎咳嗽模型豚鼠气道炎症反应和气管炎咳嗽的影响.方法 将40只豚鼠随机分为模型组、空白对照组、JZOL高剂量组、JZOL低剂量组和阿莫西林组5组,制备支气管炎咳嗽模型,给药后观察各组咳嗽敏感性;ELISA法检测血清IL-1β、CRP含量;qPCR技术测定气管组织CysLTs、MCP-1、TRPA1、CGRPmRNA水平;HE染色法观察肺组织病理变化.结果 与模型组比较,JZOL可以降低咳嗽敏感性;血清IL-1β、CRP含量虽未见显著差异,但JZOL具有明显下调趋势,其中JZOL高剂量组最低,作用强度较阿莫西林组明显;JZOL可抑制气管组织中MCP-1、CGRP、CysLTs和TRPA1的mRNA表达;JZOL对肺组织病理变化有改善作用,病变程度由高至低依次为JZOL低剂量组、阿莫西林组、JZOL高剂量组.结论 JZOL可以抑制气道炎症,缓解支气管炎咳嗽,其可能通过调控TRPA1通路发挥止咳作用.
Abstract
Objective To explore the effects of Jinzhen Oral Liquid(JZOL)on airway inflammations and bronchitis cough in model guinea pigs with bronchitis.Methods Forty guinea pigs were randomly divided into 5 groups,including a model group,a blank control group,a JZOL high-dose group,a JZOL low-dose group and an amoxicillin group.A bronchitis cough model was established in the guinea pigs.After intervention,the cough sensitivity was observed in each group,the levels of serum IL-1β and CRP were detected by ELISA,the mRNA expression levels of CysLTs,MCP-1,TRPA1,CGRP in tracheal tissues were measured by qPCR,and the pathological changes in lung tissues were observed by HE staining.Results Compared with the model group,JZOL lowered the cough sensitivity in the treatment groups.Although there was no significant difference in the levels of serum IL-1 β and CRP,JZOL showed a significant tendency in reducing their levels,among which the levels of serum IL-1 β and CRP were the lowest in the JZOL high-dose group,indicating a more significant effect than amoxicillin.JZOL inhibited the mRNA expression of MCP-1,CGRP,CysLTs and TRPA1 in tracheal tissues.In addition,JZOL improved the pathological changes of lung tissues,and the degree of lesions was ranked from high to low in the JZOL low-dose group,amoxicillin group,and JZOL high-dose group.Conclusion JZOL can inhibit airway inflammation,relieve bronchitis cough,and its mechanism on relieving cough is maybe associated with regulating the TRPA1 pathway.
基金项目
国家重点研发计划项目(2018YFC1707400)
国家科技期刊平台
NSTL
万方数据