Effect of Ershen Tang on ferroptosis in myocardial tissue of rats in a model of coronary heart disease
Objective To study the effect of Ershen Tang on ferroptosis of cardiomyocytes in rats modelled with coronary heart disease.Methods A rat model of coronary heart disease was constructed by applying vitamin D3 combined with posterior pituitary hormone with high-fat feed for 12 consecutive weeks;rats with successful modelling were randomly divided into a blank control group(Ctrl),a model group(Model),an Ershen Tang high-dose group(ESH),an Ershen Tang medium-dose group(ESM),an Ershen Tang low-dose group(ESL),and a positive drug group(Y).Conventional electrocardiogram was performed in Ⅱ leads;pathological and morphological changes of myocardial tissues of rats in each group were observed by HE and Masson staining;ELISA detected the levels of ROS,LPO,SOD,MDA,4-HNE,GSH and Fe2+in the myocardial tissues of rats in each group;RT-PCR detected the expression levels of iron-death-related genes,FTH1,SLC7A11,and GPX4 mRNA expression levels in the myocardial tissues of rats.Results Ershen Tang improved collagen fibre proliferation and reduced inflammatory cell infiltration in myocardial tissues;increased the activities of GSH and SOD(P<0.05),decreased the levels of ROS,LPO,MDA,and 4-HNE(P<0.05),and reduced the level of Fe2+(P<0.01);promoted the expression of FTH1,GPX4,and SLC7A11 mRNA(P<0.05).Conclusion Ershen Tang was able to reduce myocardial tissue damage in rats with coronary heart disease,and its mechanism may be related through inhibiting ferroptosis of cardiomyocytes.
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维普
