Study on the mechanism of fu-viscera-dredging effect of Yisui Poxue Decoction on improving intestinal motility in mice with intracerebral hemorrhage based on the SCF/C-kit signaling pathway
Objective To observe the effects of Yisui Poxue Decoction on defecation and the SCF/C-kit signaling pathway in mice with intracerebral hemorrhage,and to explore its mechanism on regulating intestinal motility in mice with intracerebral hemorrhage.Methods Fifty-four SPF healthy male C57BL/6J mice were randomly divided into a sham operation group,a model group and a prescription treatment group.An experimental intracerebral hemorrhage model was made by a rat tail autologous blood injection,while the sham operation group was only needled without an injection.The treatment group was given 1g/mL Yisui Poxue Decoction by gavage,while the sham operation group and the model group were given the same volume of normal saline,once a day.The defecation of mice in each group was observed,and the amount of defecation within 6 hours on the first,third,and seventh days,as well as the interval between the first defecation and the last gavage was recorded,and the mice were sacrificed at the same time,then,immunohistochemistry and Western Blot were used to detect the expression levels of SCF and C-kit proteins in the colon tissue of mice.Results Compared with the sham operation group,the defecation volume of mice in the model group was significantly reduced within 6 hours on the first,third,and seventh days(P<0.05),and the first defecation time after the last gavage was significantly delayed(P<0.05),the expression levels of SCF and C-kit proteins in the colon tissue were significantly reduced(P<0.05),and gradually decreased with the prolongation of the cycle.Compared with the model group,the amount of defecation in the treatment group was relatively increased within 6 hours on the first,third,and seventh days(P<0.05),the first defecation time was shortened(P<0.05),the expression levels of SCF and C-kit proteins were increased(P<0.05),and gradually recovered to normal.Conclusion The intervention of Yisui Poxue Decoction in mice with intracerebral hemorrhage can increase intestinal motility,promote defecation,promote bowel movement and play its role in promoting the bowel function recovery,whose mechanism may be related to the SCF/C-kit signaling pathway.
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