Research on the sensitivity of β-elemene combined with osimertinib to lung cancer cells based on transcriptome sequencing
Objective To investigate the in vitro pharmacological effects and mechanisms of β-elemene on the enhancement of osimertinib responsiveness to lung cancer cells.Methods The lung cancer cell viability was assessed in varying dosages of β-elemene,osimertinib,and their combinations through cell proliferation experiments.Wound healing,cell invasion test and flow cytometry were used to detect the ability of lung cancer cells in β-elemene,osimertinib and combination groups to inhibit invasion and migration and induce apoptosis.Subsequently,transcriptome sequencing analysis was applied to explore the mechanism by which β-elemene increases its sensitivity to osimertinib.Results The survival of lung cancer cells was markedly reduced when subjected to β-elemene at dosages of 50,100,200,and 400 µg/mL in conjunction with osimertinib in a gradient of concentrations including 2.5,5,10,and 20 µM(P<0.05).Furthermore,when lung cancer cells were treated with a mixture of β-elemene at 100 µg/mL and osimertinib at 5 µM,the amalgamated treatment notably suppressed cell migration and invasion(P<0.05),and also prompted apoptosis.The MAPK pathway and apoptosis-related biological processes were enriched in the transcriptome sequencing analysis of PC9 lung cancer cells.Conclusion β-elemene increases the sensitivity of osimertinib to lung cancer cells through the MAPK pathway and promotes cell senescence and apoptosis.
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