Research on the quality standard of Qianlie Guishao granules
Objective To establish the quality standard of Qianlie Guishao granules.Methods The thin layer chromatography(TLC)was used for the qualitative identification of prepared rehmannia root,peony root,peach seed,milkvetch root and amur cork-tree.The content of berberine hydrochloride was determined by high performance liquid chromatography(HPLC).Results The specificity of TLC was strong,on which the spots were clear and no negative interference was observed in Qianlie Guishao granules.The linear range of berberine hydrochloride was 0.090 1-1.6215μg(r=0.999 8),its average recovery rate was 98.54％,and the relative standard deviation(RSD)was 0.87％(n=6).Conclusion The methods of TLC qualitative identification and HPLC content determination are accurate and feasible,which can effectively control the quality of Qianlie Guishao granules.
Qianlie Guishao granulesthin layer chromatography(TLC)high performance liquid chromatography(HPLC)berberine hydrochloridequality standard
万方数据
维普
