日本鳗鲡TBK-1基因的克隆及免疫刺激的表达模式
Cloning and Expression Pofile Analysis After Immune Stimulation of TBK-1 Gene in Japanese Eel(Anguilla japonica)
赵歌洁 1黄贝 2黄文树2
作者信息
- 1. 集美大学水产学院,福建 厦门 361021
- 2. 集美大学水产学院,福建 厦门 361021;鳗鲡现代产业技术教育部工程研究中心,福建 厦门 361021;福建省水产生物育种与健康养殖工程研究中心,福建 厦门 361021
- 折叠
摘要
利用PCR技术克隆了日本鳗鲡(Anguilla japonica)TBK-1基因(AjTBK-1),其开放阅读框为2193 bp,编码731个氨基酸.序列结构分析结果显示,AjTBK-1含有4个保守结构域,分别为氨基端激酶结构域,泛素样结构域和羧基端两个卷曲-螺旋结构域.系统发育分析表明,鱼类与四足类的TBK-1各自聚为一枝.实时定量PCR(qPCR)结果显示,AjTBK-1在日本鳗鲡各组织中均有表达.Poly Ⅰ:C刺激6h后,日本鳗鲡脾脏组织中AjTBK-1的上调倍数最高,为对照组的1.63倍;迟缓爱德华氏菌(Edwardsiella tarda)感染24h后,日本鳗鲡肝脏组织中AjTBK-1的上调倍数最高,为对照组的2.2倍:表明AjTBK-1参与了日本鳗鲡抗病毒、抗细菌免疫反应应答.
Abstract
The TBK-1 gene from Japanese eel(Anguilla japonica)named AjTBK-1 was cloned using poly-merase chain reaction(PCR)technology,which has an open reading frame of 2193 bp encoding 731 amino acids.Sequence and structure analysis showed that AjTBK-1 contains four conserved domains,including an N-terminal kinase domain,a ubiquitin-like domain,and two coiled-coil domains at the C-terminus.Phyloge-netic analysis indicated that fish and tetrapod TBK-1 formed separate branches.qPCR results showed that AjT-BK-1 is expressed in various tissues of Japanese eel.After stimulation with Poly Ⅰ:C for 6 h,the maximal ex-pression of AjTBK-1 was detected in the spleen,which was 1.63-fold high than that of the control group.After infection with Edwardsiella tarda for 24 h,the maximal expression of AjTBK-1 was detected in the liver,which was 2.2-fold hign than that of the control group.Taken together,our results indicated that AjTBK-1 is involved in the antibacterial and antiviral immune responses of Japanese eel.
关键词
日本鳗鲡/TBK-1基因/序列分析/转录表达Key words
Japanese eel(Anguilla japonica)/TBK-1 gene/sequence analysis/transcriptional expression引用本文复制引用
基金项目
福建省自然科学基金项目(2021J02047)
福建省自然科学基金项目(2020J01666)
国家自然科学基金项目(32073011)
出版年
2024
国家科技期刊平台
NSTL
万方数据