Cloning and Expression Pofile Analysis After Immune Stimulation of TBK-1 Gene in Japanese Eel(Anguilla japonica)
The TBK-1 gene from Japanese eel(Anguilla japonica)named AjTBK-1 was cloned using poly-merase chain reaction(PCR)technology,which has an open reading frame of 2193 bp encoding 731 amino acids.Sequence and structure analysis showed that AjTBK-1 contains four conserved domains,including an N-terminal kinase domain,a ubiquitin-like domain,and two coiled-coil domains at the C-terminus.Phyloge-netic analysis indicated that fish and tetrapod TBK-1 formed separate branches.qPCR results showed that AjT-BK-1 is expressed in various tissues of Japanese eel.After stimulation with Poly Ⅰ:C for 6 h,the maximal ex-pression of AjTBK-1 was detected in the spleen,which was 1.63-fold high than that of the control group.After infection with Edwardsiella tarda for 24 h,the maximal expression of AjTBK-1 was detected in the liver,which was 2.2-fold hign than that of the control group.Taken together,our results indicated that AjTBK-1 is involved in the antibacterial and antiviral immune responses of Japanese eel.
Japanese eel(Anguilla japonica)TBK-1 genesequence analysistranscriptional expression
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