Gem is also a kind of mineral,and different gemstones forming processes accompany corresponding geological conditions.In addition to its beauty and rarity,the gem itself also has the char-acteristic of high chemical stability.The analysis of gemstones can also estimate the physical and chemi-cal environment,such as temperature and pressure conditions,fluid mineral substance and so on.EP-MA with its simple and convenient,as small as 1μm3 micro composition analysis,high resolution,high sensitivity and non-destructive features,as well as point analysis,line analysis and mapping distribu-tion research,has been widely used in the field of mineralogy.In this paper,the ability of the EPMA in microanalysis was demonstrated by testing several kinds of raw gemstones,such as jade,garnet,lapis lazuli and tourmaline,and also based on EPMA analysis data inferred mineral compositions and original nature forming environment of gemstones.