Orthogonal Experiment to Optimize the Extraction Process of Trisite Acid Chemical Components of Loquat Leaf
The aim of this study was to optimize the extraction process of ursolic acid and oleanol-ic acid from Eriobotrya japonica.Orthogonal test was used to determine the total concentration of ursol-ic acid and oleanolic acid in the extract.The content was determined by HPLC.The results showed that the optimal extraction process of ursolic acid and oleanolic acid from Eriobotrya japonica was:reflux ex-traction method,90％ethanol as the extraction solvent,extraction temperature 70℃,liquid-to-solid ratio 10:1,extracted once for 1h each time.This optimized process is stable and reasonable,and can ob-tain a relatively high concentration of ursolic acid and oleanolic acid.
loquat leaftripteric acidursolic acidoleanolic acidextraction and separationcontent determination
万方数据
维普
