The stage top devices for in situ cell culture and observation play an important role in the study of the real-time dynamic behavior of cells,but the research on the heat transfer characteris-tics inside such micro-devices is still incomplete.In this paper,a micro-incubator with symmetrically mounted circulating fans on the two-opposite chamber-walls is designed,and a multi-physical field simulation model is established,and the three-dimensional temperature distribution,gas convection and water vapor effect are simulated and analyzed.The results show that the liquid temperature at the bottom of the inner hole of the cell culture plate can be raised to 37℃ within 20 minutes by heating plates with a total power of 60W,and the average liquid temperature under the consideration of the heat and mass transfer effects of water vapor is more than 1.6℃ lower than the average water temperature when evaporation is ignored.
关键词
载物台细胞培养器/传热特性/水分蒸发/有限元仿真
Key words
stage-top cell culture apparatus/heat transfer characteristics/evaporation of water/finite element simulation
维普
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