To improve the extraction efficiency of alcohol soluble proteins in rice,the extraction technology was optimized using rice in the middle reaches of the Yangtze River as the research object.Control factors such as temperature and time,and analyze the extraction effect using G-250 staining method.The experimental results show that when the experimental temperature is 50℃,the extraction time is 10 hours,the centrifugal environment is set to 10000 r/min for 10 minutes,the solid-liquid ra-tio is set to 0.025g:1ml,and the extraction solution is 70％ethanol,the extraction of lysozyme protein is the optimal condition.Meanwhile,compared to the default conditions,the overall extraction effect of the optimal conditions is improved by 7.98％.From this,it can be seen that controlling the experimen-tal conditions will further improve the extraction efficiency of lysozyme proteins.The research content provides important technical support for rice variety identification and protein extraction.
关键词
长江中游/水稻/提取/醇溶蛋白/G-25/0染色法
Key words
middle reaches of the Yangtze River/rice/extract/alcohol soluble eggs/G-250 staining method
维普
万方数据