Complete Chloroplast Genome of Clematis ranunculoides:Characterization and Phylogeny Analysis
To reveal the chloroplast genome characteristics of Clematis ranunculoides and its phylogenetic relationships,the present study adopted the Illumina platform to acquire high-throughput sequences,which were used for assemble,annotate,and characterize the complete chloroplast genome of C.ranun-culoides.And then molecular phylogenetic trees were constructed with maximum parsimony(MP),max-imum likelihood(ML)and Bayesian(BI)methods.The result shows that the total length of the chloro-plast genome of C.ranunculoides is 159 741 bp,which encodes 112 genes,including 79 protein-coding genes,29 tRNA genes and 4 rRNA genes;there are 84 SSRs in the chloroplast genome of C.ranuncu-loides,and 57 of them are mono-nucleotide.Analysis of codon usage bias reveales that leucine is the most frequently utilized amino acid in the chloroplast genome.The topological structures are basically identical among MP,ML,and BI trees.Derived from the phylogenetic trees,Clematis is a monophyletic group,closely related to Anemone.And C.ranunculoides is closest to C.montana,which supportes the tradi-tional classification results.
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