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超声对猕猴桃溃疡病的抑制作用

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首先通过设置不同超声频率、超声处理时长在体外对丁香假单胞菌猕猴桃致病变种(Psa)进行处理,检测处理后Psa的活菌数、呼吸链脱氢酶活性,筛选具有较好杀菌效果的超声处理条件,然后对Psa侵染后的湘吉红、贝木离体枝条进行不同条件的超声处理,培养30 d后观测湘吉红、贝木离体枝条溃疡病病斑大小,探究不同超声条件对猕猴桃枝条溃疡病的抑制效果.结果表明,60 kHz超声处理45 min或者80 kHz超声处理15 min能够将贝木枝条病斑控制在2 cm左右,80 kHz超声处理30 min能将湘吉红枝条病斑控制在2 cm内,且能完全抑制贝木枝条的溃疡病感染.
Inhibitory Effect of Ultrasound on Kiwifruit Bacterial Canker
Pseudomonas syringae pv.actinidiae(Psa)was treated in vitro by setting different ultrasonic frequencies and ultrasonic durations.The number of viable bacteria and respiratory chain dehydrogenase activity of Psa were detected after treatment,and the ultrasonic treatment conditions with better bacteri-cidal effect were screened.Secondly,the detached branches of Xiangjihong and Beimu after Psa infection were treated with ultrasonic waves under different conditions.After 30 days of culture,the size of canker lesions was detected to explore the inhibitory effect of different ultrasonic conditions on kiwifruit canker.The results showed that 60 kHz ultrasonic treatment for 45 min or 80 kHz ultrasonic treatment for 15 min could control the lesion of Beimu branches to about 2 cm.Treatment with 80 kHz for 30 min could control the lesion of Xiangjihong branches within 2 cm,and completely inhibit the infection of can-ker spot on the Beimu branches.

kiwifruit bacterial cankerultrasonic sterilizationdisease controlPseudomonas syringae pv.actinidiae

镇熙、林诗艳、王娜灵、张棕豪、黎骏杰、谭泽成、刘世彪、查满荣、王琰

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吉首大学生物资源与环境科学学院,湖南吉首 416000

猕猴桃溃疡病 超声杀菌 病害防治 丁香假单胞菌猕猴桃致病变种

2024

吉首大学学报(自然科学版)
吉首大学

吉首大学学报(自然科学版)

影响因子:0.451
ISSN:1007-2985
年,卷(期):2024.45(3)