Purification Process of Total Flavonoids from Lilium lancifolium Thunb and Its Antibacterial Activity
Through comparing the static adsorption-desorption performance of 7 kinds of macroporous resins,the best macroporous resin with high adsorption rate and desorption rate was selected.The opti-mal purification process of Lilium lancifolium by macroporous resin was selected by single factor experi-ment and orthogonal experiment,and the total flavonoid content and antibacterial activity before and af-ter purification were determined.The results showed that the macroporous resin with high adsorption and desorption performance was AB-8.The optimal purification process was as follows:the total fla-vonoid concentration of the feed solution was 0.076 4 g/L,the pH value was 4,the adsorption time was 8 hours,the solvent volume fraction was 60％,the solvent volume was 120 mL,and the desorption time was 10 hours.The total flavonoid content after purification was 11.55％,which was 5.3 times that of the purification before.The inhibition zone diameter of the purified total flavonoid was larger than that of the total flavonoid before purification,and the inhibitory effect on Staphylococcus aureus was higher than that on Escherichia coli.
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