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水肥耦合对设施番茄叶片酶活性及水肥利用的影响

Effects of Water and Fertilizer Coupling on Enzyme Activity and Water and Fertilizer Utilization in Greenhouse Tomato Leaves

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探究水肥耦合对以蛭石为主复合基质栽培番茄叶片抗氧化酶(SOD、POD、CAT)活性、叶片丙二醛(MDA)含量、脯氨酸(Pro)含量、可溶性糖(SS)含量、蛋白质(SP)含量及水分利用效率,肥料偏生产力的影响,以期为设施番茄在以蛭石为主复合基质上的栽培提供理论依据.以双赢先锋番茄为试材,试验设置灌水量、氮、磷、钾4个因素,采用四因素五水平二次正交旋转组合设计的二分之一执行.结果表明:番茄叶片SOD、CAT活性和Pro、SS含量在生育期内呈先上升后下降的趋势,POD活性和MDA、SP含量表现为上升趋势.灌水量和施肥量的过高或过低均会导致MDA含量的快速增加,使酶活性降低.适宜的灌水量下,施中等水平肥能得到较高的POD、CAT、SS含量,施较高氮肥能得到较高的SP含量.在中等水平的水肥下,灌溉水利用率提升了31.93%,肥料偏生产力提高了42%.W18处理为最优处理,即灌水水平78.0%下灌水量为6 485 m3/hm2,施氮量340 kg/hm2,施磷量185 kg/hm2,施钾量310 kg/hm2,得到最大产量147.69 t/hm2,最高灌溉水利用率22.74 kg/m3,最高偏肥料生产力176.24 kg/kg.研究结果可为在以新疆产蛭石为主的复合基质中提高水肥利用率及番茄高效优质栽培水肥科学管理提供理论依据.
Studying the effects of water and fertilizer coupling on the activities of antioxidant enzymes(SOD,POD,CAT),the content of malondialdehyde(MDA),proline(Pro),soluble sugar(SS),protein(SP),and water use efficiency(WUE)of tomato leaves in the vermiculite-based composite substrate,can provide a theoretical basis for the cultivation of protected tomato on vermiculite-based composite substrate.This study employed Double Win Pioneer tomatoes as the experimental material.The experimental design included four factors:irrigation,nitrogen,phosphorus,and potassium.A half-fractional execution of a four-factor,five-level orthogonal rotated combination design was used.The results showed that the activities of SOD,CAT,and the contents of Pro,SS in tomato leaves increased first and then decreased,while the activities of POD and the contents of MDA,SP increased.Too high or too low an amount of irrigation and fertilization will lead to a rapid increase in MDA content and a decrease of enzyme activity.Under proper irrigation,the content of POD,CAT,and SS was higher with medium-level fertilizer,and the content of SP was higher with high nitrogen fertilizer.Under medium levels of water and fertilizer,water use efficiency increased by 53%,and fertilizer partial productivity increased by 42%.In this experiment,W18 was the best treatment,78.0%irrigation level,340 kg/hm2 of nitrogen,185 kg/hm2 of phosphorus,310 kg/hm2 of potassium.The maximum yield was 147.69 t/hm2,and the highest water use efficiency was 22.74 kg/m3,the highest partial fertilizer productivity was 176.24 kg/kg.This study can provide a theoretical basis for scientific management of water and fertilizer for the full use of vermiculite production and high-efficiency and high-quality tomato cultivation in Xinjiang.

Tomatowater-fertilizer couplingleafenzyme activityIrrigation water use efficiencypartial fertilizer productivity

祝洋、刘志应、李新苗、张娟

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塔里木大学园艺与林学学院,新疆 阿拉尔 843300

南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室,新疆 阿拉尔 843300

南疆设施农业重点实验室,新疆 阿拉尔 843300

番茄 水肥耦合 叶片 酶活性 灌溉水利用率 肥料偏生产力

2018年度兵团财政项目

2018BB046

2023

节水灌溉
中国国家灌溉排水委员会,中国灌溉排水发展中心,武汉大学,国家节水灌溉北京工程技术研究中心

节水灌溉

CSTPCD北大核心
影响因子:0.674
ISSN:1007-4929
年,卷(期):2023.(12)
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