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干旱胁迫和复水对火麻生理及次生代谢产物含量的影响

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为探究火麻干旱胁迫的耐受能力及复水后其次生代谢产物的含量变化.以土培盆栽的方式培养火麻幼苗,以正常浇水的火麻为对照(CK),对比分析胁迫组在胁迫后的0、3、5、7、9 d和复水后的生理特性、光合及次生代谢产物的变化情况.结果表明:干旱胁迫条件下,火麻叶中含水量、光合指标、最大光能转换效率、实际光能转换效率、电子传递速率RET、光化学淬灭系数Qp降低;而非光化学淬灭系数QNP、叶绿素和类胡萝卜素、脯氨酸、可溶性蛋白、可溶性糖及MDA含量和相对电导率上升,4种抗氧化酶活性升高,复水后叶片含水量、叶绿素b、光合指标、脯氨酸含量和SOD活性低于CK组,其他各生理指标均高于CK组水平.总黄酮、总酚和总萜含量升高,复水后这3种次生代谢产物含量不同程度地低于对照.火麻的第1、2对叶片中CBD含量随胁迫程度的增加而降低,且远高于下部叶片;第3、4对叶中CBD含量虽在7 d胁迫处理时含量最高,但仍低于对照并且2者差异不显著(P<0.05);第5、6对叶片在胁迫处理的3 d时CBD含量达到峰值.复水后,3组叶位中的CBD含量均高于CK组.综合研究发现,适度的干旱胁迫会提高火麻的生理抗逆性,降低其光合作用,提高次生代谢产物的含量;胁迫会降低药物活性物质CBD的含量;在适度的胁迫经复水后,缓解了胁迫对植物的伤害,并提高叶片中CBD的含量.
Effects of Drought Stress and Rewatering on Physiology and Contents of Secondary Metabolites of Cannabis sativa L.
In order to understand the tolerance to drought stress of Flax vulgaris and content changes of secondary metabolites of Flax vulgaris after rehydration.The seedlings C.sativa were cultured in the pot,normal watering flax was taken as control(CK),the changes of physiological characteristics,photosynthesis,and secondary metabolites in control and stress groups at 0,3,5,7,and 9 days after stress and after rehydration were studied.The results showed that water content,photosynthetic index,maximum light energy conversion efficiency,actual light energy conversion efficiency,RET and Qp decreased under drought stress.The contents and relative electrical conductivity of QNP,chlorophyll and carotenoid,proline,soluble protein,soluble sugar,and MDA increased,and the activities of four antioxidant enzymes increased.After rehydration,water content,chlorophyll b,photosynthetic indexes,proline content,and SOD activity of leaves were lower than those of the CK group,while other physiological indexes were higher than those of the CK group.After rehydration,the contents of these three secondary metabolites were lower than those of the CK.The CBD content in the first and second pair leaves of flax fire decreased with the increase of stress degree,and was much higher than that in the lower leaves.The CBD content in leaves of the third and fourth pairs was the highest at 7 d of stress treatment,but it was still lower than that of CK and there was no significant difference between them(P<0.05).The content of CBD in leaves of the fifth and sixth pairs reached the peak at 3 d after stress treatment.After rehydration,CBD content in leaves of 3 groups was higher than that of the CK group.The comprehensive study showed that drought stress could improve physiological stress resistance,reduce photosynthesis,and increase the content of secondary metabolites.Stress can reduce the content of CBD;After a certain degree of stress exercise,the damage to plants was reduced and the content of CBD was increased.

Cannabis sativadrought stresswater stressphysiological charactersphotosynthetic characteristicssecondary metaboliterewateringdeficit irrigation

刘同歌、苑海鹏、叶云舒、张一宁、屈施旭、张玉红

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东北林业大学森林植物生态学教育部重点实验室,哈尔滨 150040

黑龙江省林源活性物质生态利用重点实验室,哈尔滨 150040

火麻 干旱胁迫 水分胁迫 生理特性 光合特性 次生代谢产物 复水 亏水灌溉

森林植物生态学教育部重点实验室(东北林业大学)开放基金

KF2022013

2023

节水灌溉
中国国家灌溉排水委员会,中国灌溉排水发展中心,武汉大学,国家节水灌溉北京工程技术研究中心

节水灌溉

CSTPCD北大核心
影响因子:0.674
ISSN:1007-4929
年,卷(期):2023.(12)
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