Effects of Drought Stress and Rewatering on Physiology and Contents of Secondary Metabolites of Cannabis sativa L.
In order to understand the tolerance to drought stress of Flax vulgaris and content changes of secondary metabolites of Flax vulgaris after rehydration.The seedlings C.sativa were cultured in the pot,normal watering flax was taken as control(CK),the changes of physiological characteristics,photosynthesis,and secondary metabolites in control and stress groups at 0,3,5,7,and 9 days after stress and after rehydration were studied.The results showed that water content,photosynthetic index,maximum light energy conversion efficiency,actual light energy conversion efficiency,RET and Qp decreased under drought stress.The contents and relative electrical conductivity of QNP,chlorophyll and carotenoid,proline,soluble protein,soluble sugar,and MDA increased,and the activities of four antioxidant enzymes increased.After rehydration,water content,chlorophyll b,photosynthetic indexes,proline content,and SOD activity of leaves were lower than those of the CK group,while other physiological indexes were higher than those of the CK group.After rehydration,the contents of these three secondary metabolites were lower than those of the CK.The CBD content in the first and second pair leaves of flax fire decreased with the increase of stress degree,and was much higher than that in the lower leaves.The CBD content in leaves of the third and fourth pairs was the highest at 7 d of stress treatment,but it was still lower than that of CK and there was no significant difference between them(P<0.05).The content of CBD in leaves of the fifth and sixth pairs reached the peak at 3 d after stress treatment.After rehydration,CBD content in leaves of 3 groups was higher than that of the CK group.The comprehensive study showed that drought stress could improve physiological stress resistance,reduce photosynthesis,and increase the content of secondary metabolites.Stress can reduce the content of CBD;After a certain degree of stress exercise,the damage to plants was reduced and the content of CBD was increased.