The Effect of Organic Fertilizer Combined with Biochar on the Fungal Community Structure in Alkalized Soil
To explore the reshaping effect of combined application of organic fertilizer and biochar on reshaping the fungal community structure in alkaline soils of arid regions,field experiments were conducted using high-throughput sequencing technology to investigate the influences of various levels of organic fertilizer(0,7.5 t/hm2)and biochar(0,2%,and 4%)on soil fungal community structure and crop yield.The results showed that,compared with the control,the combination treatment of 7.5 t/hm2 organic fertilizer and 2%and 4%biochar reduced the relative abundance of Ascomycota at the phylum level,and beneficial microbiota increased,such as Basidiomycota and Glomeromycota.The Observed index and Shannon index of soil fungi increased,significantly,while the Simpson index decreased.The compound treatment improved the richness,diversity and community structure of soil fungi,which was beneficial for the growth and yield of oil sunflower.The yield of 7.5 t/hm2 organic fertilizer combined with 4%biochar was the highest,reaching 2 521.5 kg/hm2,an increase of 1 589.1 kg/hm2 compared to the control.Therefore,compared to using a single material,the combination of organic fertilizer and biochar can further improve the structure and diversity of soil fungal communities,and increase crop yields.