Simultaneous Determination of 44 Antibiotic in 5 Categories in Groundwater by Solid-phase Extraction-liquid Chromatography-tandem Mass Spectrometr
A method combining solid-phase extraction with liquid chromatography-tandem mass spectrometry was developed for the detection of 44 antibiotics in groundwater.The optimal parameters were as follows:the water was eluted at a rate of 2 mL/min with 5 mL of 0.1%formate-methanol and 3 mL of 5%ammonia-methanol by adjusting the pH of the sample to 3 and adding 0.5 g/L Na2EDTA.The samples were quantified using dynamic multiple-reaction monitoring mode.The antibiotics showed satisfactory linearity within the 0～50.0 μg/L range,with linear correlation coefficients all greater than 0.990.The method detection limits ranged from 0.05 to 0.9 ng/L.The average recoveries of the antibiotics were in the range of 39.1%～143%,with relative standard deviations(RSD)ranging from 1.9%to 14%.Finally,the optimized method was successfully applied to analyze ground water samples collected from northern Jiangsu.The results indicated that the 18 antibiotics could be detected at concentrations ranging from not detected(ND)to 21.7 ng/L.
Liquid chromatography-tandem mass spectrometry(LC-MS/MS)Solid phase extraction(SPE)AntibioticsGroundwater
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