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EPS及消毒剂对细菌表面附着和初期生物膜形成的影响

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为探究胞外聚合物(extracellular polymeric substances,EPS)和消毒剂对细菌表面附着聚集行为的影响,文中以野生型铜绿假单胞菌和藻酸盐分泌过量的突变株为研究对象,分析不同氯浓度条件下细菌EPS生成及其对表面附着和初期生物膜形成的影响。结果表明,相较于野生型,突变型的生物膜形成优势显著,特别是当氯质量浓度≥1。0 mg/L时,强化了其形成优势;EPS也显著提高了生物膜的黏附强度以及降低其表面粗糙程度。低浓度氯促进细菌EPS的生成以及初期生物膜的形成,但高质量浓度氯(3。0 mg/L)则抑制了生物膜的形成,同时也降低了两种菌株生物膜的黏附力与表面粗糙度。对于两种菌株而言,聚氯乙烯(PVC)管材上生物膜的形成优势显著高于不锈钢(STS)管材。
Effects of EPS and Disinfectants on Bacterial Surface Adhesion and Initial Biofilm Formation
To investigate the effects of EPS and disinfectants on bacterial surface attachment aggregation behavior,wild-type Pseudomonas aeruginosa and alginate over-secreting mutant strain were used in this paper to analyze bacterial EPS production under different chlorine concentration conditions and its relationship.The results showed that the biofilm formation advantage of mutant strain was significant compared to wild-type,especially when the chlorine concentration was≥1.0 mg/L,which enhanced its formation advantage.Also EPS significantly increased the adhesion strength of biofilm and reduced its surface roughness.Low chlorine concentrations promoted bacterial EPS production and initial biofilm formation,but high chlorine concentrations(3.0 mg/L)inhibited biofilm formation and also reduced biofilm adhesion and surface roughness for both strains.For both strains,the advantage of biofilm formation on PVC pipe was significantly higher than that on stainless steel pipe.

disinfectantbiofilmextracellular polymeric substance(EPS)surface adhesionbiofilm roughness

钟疏影、余超、陈国炜、蔡寅诺、刘丽

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合肥工业大学土木与水利工程学院,安徽合肥 230009

消毒剂 生物膜 胞外聚合物(EPS) 表面附着 生物膜粗糙度

安徽省自然科学基金"水科学"联合基金项目&&

2208085US13

2024

净水技术
上海市净水技术学会,上海市城乡建设和交通委员会科学技术委员会办公室

净水技术

CSTPCD
影响因子:0.643
ISSN:1009-0177
年,卷(期):2024.43(2)
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