Effects of EPS and Disinfectants on Bacterial Surface Adhesion and Initial Biofilm Formation
To investigate the effects of EPS and disinfectants on bacterial surface attachment aggregation behavior,wild-type Pseudomonas aeruginosa and alginate over-secreting mutant strain were used in this paper to analyze bacterial EPS production under different chlorine concentration conditions and its relationship.The results showed that the biofilm formation advantage of mutant strain was significant compared to wild-type,especially when the chlorine concentration was≥1.0 mg/L,which enhanced its formation advantage.Also EPS significantly increased the adhesion strength of biofilm and reduced its surface roughness.Low chlorine concentrations promoted bacterial EPS production and initial biofilm formation,but high chlorine concentrations(3.0 mg/L)inhibited biofilm formation and also reduced biofilm adhesion and surface roughness for both strains.For both strains,the advantage of biofilm formation on PVC pipe was significantly higher than that on stainless steel pipe.