净水技术2024,Vol.43Issue(2) :90-96.DOI:10.15890/j.cnki.jsjs.2024.02.011

磁环境下AAO工艺中的微生物群落演替规律

Succession Regulations of Microflora in AAO Process under Magnetic Environment

王越 王嘉斌 赵培海 宋健
净水技术2024,Vol.43Issue(2) :90-96.DOI:10.15890/j.cnki.jsjs.2024.02.011

磁环境下AAO工艺中的微生物群落演替规律

Succession Regulations of Microflora in AAO Process under Magnetic Environment

王越 1王嘉斌 1赵培海 2宋健3
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作者信息

  • 1. 济南大学土木建筑学院,山东济南 250002
  • 2. 利津县胜瑞城市建设集团有限公司,山东东营 257400
  • 3. 山东省环境保护科学研究设计院有限公司,山东济南 250109
  • 折叠

摘要

将AAO反应器置于5 mT大小的平均磁场环境,文中研究了磁场对AAO工艺效能的影响,并从分子生物学的角度对其影响机理进行了初步探究.结果表明,在 5 mT磁场环境下,AAO工艺启动阶段的平均总氮去除率有较明显提升,比对照组上升 6%;AAO反应器启动时间较对照组缩短了 5 d;AAO中以固氮螺旋菌(Azospira)为代表的反硝化菌群的相对丰度占比明显提升,丝硫菌属(Thiothrix)的相对丰度占比大幅降低,对污泥膨胀的抑制作用较明显;通过基因功能预测发现磁环境下细胞运动类基因相对丰度相对无磁场条件提升 75%,膜运输类基因大幅下降,从而提高了AAO的启动速度,提升了脱氮效能.

Abstract

The effect of magnetic field on the performance of AAO process was studied by placing AAO reactor in an average magnetic field environment of 5 mT,and mechanism of its effect was preliminarily explored from the perspective of molecular biology.The results showed that under a magnetic field environment of 5 mT,the average total nitrogen removal rate in start-up stage of AAO process was significantly improved,with an increase of 6%compared to the control group;and start-up time of AAO reactor was shortened by 5 days compared with control group.The relative abundance of denitrifying bacteria represented by Azospira in AAO was significantly increased,relative abundance of Thiothrix was greatly reduced,and inhibitory effect on sludge bulking was obvious.Through gene function prediction found that relative abundance of cell movement genes in magnetic environment increased by 75%compared with that in control group,and membrane transport genes decreased significantly,thereby startup speed and denitrification efficiency of AAO were significantly improved.

关键词

磁场/AAO工艺/生物脱氮/群落结构/功能基因预测

Key words

magnetic field/AAO process/biological nitrogen removal/microflora structure/functional gene prediction

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基金项目

国家自然科学基金项目(52100088)

济南大学科技计划项目(XBS2001)

出版年

2024
净水技术
上海市净水技术学会,上海市城乡建设和交通委员会科学技术委员会办公室

净水技术

CSTPCD
影响因子:0.643
ISSN:1009-0177
参考文献量2
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