Comparative Analysis of Enzyme Substrate Spectrometry and Enzyme Substrate Method for Determination of Coliform Bacteria in Water
Simultaneous determination of total coliforms,Escherichia coli,and fecal coliforms in different types of water samples was carried out using novel enzyme substrate spectrometry and enzyme substrate method.Both methods have their own advantages.After processing the detection results using SPSS software,paired t-tests were conducted.The results showed that the P value of the two groups of determination of total coliforms was 0.057,and the correlation coefficient was 0.904.The P value of two groups of Escherichia coli was 0.593,and the correlation coefficient was 0.972.The P value of the two groups of fecal coliforms was 0.136,and the correlation coefficient was 0.986.It indicated a correlation between the two detection methods,and there was no statistically significant difference.The enzyme substrate spectrometry method was used for detecting certified quality control samples,and the detection results of determination of total coliforms,Escherichia coli,and fecal coliforms were all within the true value range of the quality control sample,proving that the detection accuracy of this method met the requirements.Compared with enzyme substrate method,enzyme substrate spectrometry has similar reaction principles and is more convenient to operate.It has advantages such as instrument automation and intelligent rapid detection,interpretation,storage,and online transmission of data.It can meet the needs of routine laboratory testing and has strong application prospects and advantages in emergency on-site testing.
万方数据
维普
