Implementation of Quick Start-Up for Anammox Reactor by Stratified Inoculation
The Anammox reactor by inoculating traditional biological sludge starts for a long time and even fails to start.Mixed with Anammox sludge,traditional biological sludge can release ammonia nitrogen and organic matter to inhibit Anammox bacteria,the low activity of the bacteria slows down the start-up of the reactor.Therefore,the innovative strategy of layered inoculation of traditional biological sludge and Anammox sludge was proposed.The start-up characteristics of the reactors with layered inoculation of activated sludge(upper layer)and Anammox granular sludge(lower layer)and the enrichment of Anammox bacteria by activated sludge were investigated.On thirteenth day of operation,opening the reflux generated a short-term impact,and the reaction period quickly recovered and stabilized,and the Anammox reactor was successfully started.On the 86th day of operation,the SAA of activated sludge in the upper layer was 2.5 mg NH4+/(g VSS·d).After 144 days cultivation,the NRR reached 10.8 kg N/(m3·d),the excess sludge contained 28.2%Candidatus_Brocadia and 1.5%Candidatus_Jettenia,the copy number of Anammox attained 8.7×1016 g-1.Stable and efficient Anammox reactor has been started successfully.