Systematic Evaluation of Uncertainty for Algae Detection in Source Water by Visual Field Counting Method
In this paper,a systematic method for evaluating the uncertainty of test results to address the challenges of quality control was developed in algae counting laboratories and the need for highly qualified test personnel.The study systematically analysed the uncertainty sources of the detection results of algae in source water using the field of view counting method.The study counted and fixed the number of algae in the concentrated sample through a microscope.Six key influencing factors were identified and the uncertainty components they introduced were calculated.An uncertainty evaluation model for algae counting measurement results was constructed by comprehensively analysing the uncertainty propagation law.A method was established to evaluate the uncertainty of algae counting results in source water.The uncertainties introduced by sampling,fixation,constant volume,sectioning,microscopy,and repeatability measurement in the process of algae counting detection were 0.004 08,0.002 45,0.004 08,0.000 081 9,0.007,and 0.019 8,respectively.The results of algae counting detection were mainly affected by repeatability measurement.The concentration of algae in the sample had a significant impact on the uncertainty of counting results.If the detection result was less than 1 million L-1,the expanded uncertainty of the detection result increased significantly.The uncertainty of detection results can be effectively controlled and the credibility of experiments improved by using instruments and equipment with high accuracy and precision,standardised operation and regular calibration.Optimising the key steps of algal counting methods is essential to improve the value of the results obtained.
algaevisual field counting methoduncertaintysource watersystematic evaluation
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