Application Analysis of a New Method for Determination of Total Coliform and Escherichia coli in Water
In order to deal with water quality emergencies,the detection time is further reduced and the detection efficiency is improved.In this paper,the detection accuracy and detection time of automatic microbiological detection analyzer method were targeted researched,and the results of the raw water sample detected by the national standard detection methods(enzyme substrate method and filter membrane method)were compared and analyzed.The automatic microbiological detection analyzer method was used to detect the high and low concentrations of quality control samples,and it was found that all the test results met quality control truth value requirements,the results of this method were reliable.Using serial dilutions and recording of test results and time,it was found that the detection time was from 2 to 18 hours with the sample concentration from 0 to 1.0×10 8 CFU/(100 mL),the higher the sample concentration,the shorter the detection time.The comparison of raw water samples showed that,the automatic microbiological detection analyzer method paired with the detection results of the national standard detection method(filter membrane method and enzyme substrate method),and the paired t-test results of total coliform were P=0.122(＞0.05)and P=0.351(＞0.05),with no significant difference;meanwhile the paired t-test results of Escherichia coli were P=0.086(＞0.05)and P=0.082(＞0.05),with no significant difference.On the premise of ensuring data accuracy,the detection time can be greatly shortened by using the automatic microbiological detection analyzer method,which is suitable for detecting all kinds of emergency water quality conditions.
total coliformEscherichia coliautomatic microbiological detection analyzermembrane filtration methodenzyme substrate method
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