Simultaneous Determination of 17 Antibiotics in Water by Online Solid-Phase Extraction,High-Performance Liquid Chromatography and Tandem Quadrupole Mass Spectrometry(SPE/UPLC/MS/MS)
In this study,it was established an online enrichment high-performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)method for simultaneous detection of 17 antibiotics in water,including macrolides,sulfonamides,tetracyclines,and quinolones.The water samples were enriched online through the Waters SPE mode using the Waters Oasis® HLB solid-phase extraction cartridge(30 mm×2.1 mm,20 μm)and then separated on the ACQUITY UPLC® BEH C18 column(100 mm×2.1 mm,1.7 μm)with 0.1％formic acid aqueous solution-acetonitrile as the mobile phase.Detection in positive ion mode using multiple reaction monitoring(MRM)in an electrospray ionization source.The results indicated a good linear relationship between the target compound and mass concentration(R＞0.990)in the range of 5～100 ng/L,and the detection limit was 2.04～3.30 ng/L.The recovery rates of the 17 kinds of antibiotics were 49.1％～133.8％,and the relative standard deviation(RSD,n=6)was 3.8％～11.3％.The method was applied to detect the water samples from the drinking water sources in Wenzhou City,and except for certain reservoir downstream of erythromycin,clarithromycin,azithromycin,sulfamethoxazole,roxithromvcin,no other 12 kinds of antibiotics were detected.
liquid chromatography-tandem mass spectrometrydetectionin-situ enrichmentantibioticswater bodies
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