Evaluation of Pathogenic Bacterial Diversity and Health Risks in Urban WWTPs
A large number of pathogenic bacteria are commonly found in wastewater,and wastewater treatment plants(WWTPs),as the main sites for receiving and treating wastewater,play an important role in controlling pathogenic bacterial contamination and ensuring the safety of reclaimed water.In this study,the bacterial community structure and the presence of pathogenic bacteria in a WWTP in northern China during summer and winter were analyzed using 16S rRNA sequencing and real-time fluorescence quantitative PCR techniques.The results showed that a total of 27 pathogen-containing bacterial genera were detected,among which Pseudomonas and Acinetobacter were the main pathogenic genera.After a series of process treatment,the number and content of pathogenic genera in the effluent were significantly reduced,but it was found that the conventional effluent testing index Escherichia coli could not indicate the presence and risk level of other pathogenic bacteria,suggesting that new testing indices still need to be introduced to reduce the risk of pathogenic bacteria in reclaimed water reuse.