Visible spectroscopy combined with partial least squares method determination of free amino acids in milk at the same time
The sensitive color reaction of glycine, glutamic acid, valine and phenylalanine with copper sulfate to form a blue complex, due to the maximum absorption wavelength close to the absorption spectra overlap more serious, traditional spectrophotometry is difficult to Determination of single components. By measuring the absorption spectra of the mixed sample of glycine, glutamic acid, valine and phenylalanine analog in the 660 nm~750 nm wavelength range, the use of partial least squares method to establish the calibration model to predict its content, combined with spectrophotometry to establish a new method for simultaneous determination of amino acid content, the method is applied to the simultaneous determination of glycine, glutamic acid, valine and phenylalanine content in the analog-mixed samples and milk samples. Analog mixed sample, the recovery rate of 98.46 %~98.79 %; RPEs 1.67 %~2.34 %.
partial least squares methodspestrophotometrysimultaneous determinationmilkfree amino acids
NETL
NSTL
万方数据
维普
